Comparison of Six Culture Methods for Salmonella Isolation from Poultry Fecal Samples

Culture methods differ on the isolation of Salmonella enterica serotypes from naturally contaminated swine fecal samples.

Four culture methods (A, B, C, and D) were comparatively evaluated for their ability to isolate Salmonella enterica from pooled swine fecal samples (n = 100). None of the methods was able to isolate Salmonella from all positive samples. The relative sensitivity of the culture methods evaluated was 82%, 94%, 95%, and 78% for methods A, B, C, and D, respectively. The comparison of sensitivities s...

Comparison of Culture Media for the Isolation of Salmonella Spp. from Stool Samples

Salmonella detection in poultry samples. Comparison of two commercial real-time PCR systems with culture methods for the detection of Salmonella spp. in environmental and fecal samples of poultry.

STUDY The efficiency of two commercial PCR methods based on real-time technology, the foodproof® Salmonella detection system and the BAX® PCR Assay Salmonella system was compared to standardized culture methods (EN ISO 6579:2002 - Annex D) for the detection of Salmonella spp. in poultry samples. MATERIAL AND METHODS Four sample matrices (feed, dust, boot swabs, feces) obtained directly from p...

Isolation, Molecular Identification, and Antibiotic Resistance Profile of Salmonella Typhimurium Isolated from Calves Fecal Samples of Dairy Farms in Hamedan

Introduction: Calf diarrhea is one of the significant problems in dairy farms associated with treatment costs and reduced livestock production. Salmonellae are among the most common and the major causative agents of diarrhea in calves and humans. The present study was carried out to isolate and identify Salmonella in fecal samples of calves in industrial dairy farms of Hamedan and to determine ...

Microbial-Resistant Salmonella enteritidis Isolated from Poultry Samples

Background: Multidrug resistance in Salmonella enteritidis isolates is a public health problem worldwide; the present study, therefore, was designed for antimicrobial-resistance determination in this strain. Methods: Salmonella strains isolated from poultry samples by biochemical positive and negative tests were subjected to PCR and identified as Salmonella enteritidis. For detection and ide...

Isolation of Salmonellae from Food Samples. Vi. Comparison of Methods for the Isolation of Salmonella from Egg Products.

THE RECOVERY OF SALMONELLAE FROM EGG PRODUCTS WAS STUDIED, BY USE OF THREE DIFFERENT ENRICHMENT PROCEDURES: (i) selenite broth, (ii) selenite broth containing 10% sterile feces, and (iii) the lactose pre-enrichment procedure. Brilliant Green Agar was used throughout as the recovery medium. Although the lactose pre-enrichment methodology promoted Salmonella recovery from samples containing small...